Accession Number : ADA140459

Title :   Platelet Size Does Not Correlate with Age.

Descriptive Note : Technical rept.,


Personal Author(s) : Thompson,C B ; Love,D G ; Quinn,P G ; Valeri,C R

PDF Url : ADA140459

Report Date : 10 Mar 1983

Pagination or Media Count : 31

Abstract : The relationship between platelet size and in vivo aging was investigated in the baboon using size-dependent platelet subpopulations separated by counterflow centrifugation. The separation characteristics, size, LDH activity, and dense body content of the baboon platelet subpopulations were similar to those previously observed in studies of human platelets. Three independent labeling techniques were used: (1) in vivo labeling with 75 Se-methionine; (2) in vitro labeling with 51 Cr; and (3) in vivo labeling with 14 C-serotonin. Maximal incorporation of all three labels showed a close correlation between the mean platelet volume (MPV) of each fraction and the platelet radioactivity. The onset of incorporation and rate of accumulation of 75 Se-methionine were comparable in all fractions when corrected for differences in volume, suggesting that platelet size heterogeneity was present from the time of release of the platelets from the bone marrow.

Descriptors :   *Blood platelets, Sizes(Dimensions), Baboons, Life span(Biology), Separation, In vivo analysis, Labeled substances, Methionine, Serotonin, In vitro analysis, Chromium, Carbon, Radioactive isotopes, Radioactivity, Bone marrow, Aging(Physiology), Selenium

Subject Categories : Anatomy and Physiology
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE