Accession Number : ADA180320

Title :   Nonlinear Laser Spectroscopy Studies of Enzyme Interactions.

Descriptive Note : Annual rept. 1 Feb 86-1 Feb 87,

Corporate Author : MICHIGAN UNIV ANN ARBOR

Personal Author(s) : Steel,Duncan G ; Gafni,Ari

PDF Url : ADA180320

Report Date : 06 May 1987

Pagination or Media Count : 8

Abstract : The thrust of this project is to develop and apply new methodologies based on nonlinear optics to the study of biological macromolecules and membranes. In the period covered by this report we developed several biochemical experimental systems to which the spectroscopic techniques are being applied. The biological problems addressed include: a. Age related modifications in enzyme catalysis. 2. Mechanisms of control of membrane-bound transport proteins. The spectroscopic studies involved two primary sets of measurements based on backward nearly degenerate four-wave mixing and employing both time domain and frequency domain and frequency domain measurements. A CW dye laser system using cross-correlated optical fields was applied to measure relaxation rates of nonradiating states like nonphosphorescent triplet states and is being adapted for studies of biological systems. A laboratory for ultrafast time-domain measurements is being developed to study enzyme and membrane mechanisms. A system based on phase conjugate optics designed to eliminate the effects of light scattering in optical spectroscopy studies of biological membrane systems is being adapted for studies of biological systems. A laboratory for ultrafast time-domain measurements is being developed to study enzyme and membrane mechanisms. A system based on phase conjugate optics designed to eliminate the effects of light scattering in optical spectroscopy studies of biological membrane systems is being tested.

Descriptors :   *CATALYSIS, ENZYMES, MEMBRANES(BIOLOGY), *SPECTROSCOPY, BIOMOLECULES, MACROMOLECULES, BIOLOGY, LIGHT SCATTERING, FREQUENCY, RATES, RELAXATION, CONTINUOUS WAVE LASERS, DYE LASERS, LASERS, INTERACTIONS, MEASUREMENT, MEMBRANES, OPTICS, TIME DOMAIN, NONLINEAR SYSTEMS, HIGH RATE

Subject Categories : Biochemistry
      Organic Chemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE