Accession Number : ADA180669

Title :   Isolation of Japanese Encephalitis Virus From Clinical Specimens Using a Continuous Mosquito Cell Line,

Corporate Author : ARMED FORCES RESEARCH INST OF MEDICAL SCIENCES APO SAN FRANCISCO 96346

Personal Author(s) : Leake,Colin J. ; Burke,Donald S. ; Nisalak,Ananda ; Hoke,Charles H.

Report Date : 1986

Pagination or Media Count : 6

Abstract : During the 1983 Japanese encephalitis (JE)epidemic in northern Thailand, we systematically attempted to isolate JE virus (JEV) from clinical specimens collected from 49 consecutive JE patients at 1 provincial hospital. Fresh acute plasma and cerebrospinal fluid (CSF) samples and postmortem brain samples were immediately inoculated onto cultured monolayers of Aedes pseudoscutellaris (LSTM-AP-61)cells which had been shipped to the epidemic site. None of 49 plasma samples yielded virus. None of 30 fresh CSF samples from nonfatal cases yielded virus, but 5 of 15 (33%) CSF samples from fatal cases did. Inoculation of fresh brain specimens obtained at autopsy yielded virus in every case attempted (7 of 7), whereas postmortem needle biopsy specimens of brain yielded virus in only 1 of 4 cases. Isolates were most frequently successful using thalamic tissue (6 of 7 cases), but isolates were also commonly obtain from frontal cortex (4/7), occipital cortex (4/7), cerebellum (4/7) and pons (2/7).

Descriptors :   *JAPANESE ENCEPHALITIS VIRUSES, *ISOLATION, AEDES, AUTOPSY, BIOPSY, BRAIN, CELLS, CEREBROSPINAL FLUID, CLINICAL MEDICINE, CULICIDAE, EPIDEMIOLOGY, INOCULATION, LAYERS, NEEDLES, NORTH(DIRECTION), PLASMAS(PHYSICS), REPRINTS, SAMPLING, SITES, THAILAND, PATHOLOGY, CULTURES(BIOLOGY)

Subject Categories : Microbiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE