Accession Number : ADA181440

Title :   World Reference Center for Arboviruses.

Descriptive Note : Annual progress rept. 1 Jan-31 Dec 82,

Corporate Author : YALE UNIV NEW HAVEN CONN SCHOOL OF MEDICINE

Personal Author(s) : Shope,Robert E

PDF Url : ADA181440

Report Date : Jan 1983

Pagination or Media Count : 89

Abstract : Revisions were made of the genera Phlebovirus, Orbivirus, Bunyavirus, and the family Rhabdoviridae. New viruses of the Kemerovo and Sakhalin serogroups were identified. Serological evidence of phlebovirus antibody was found in sera from Greece, and of flavi- alpha- and bunyaviruses in sera from the Sudan. Zinga virus was identified as Rift Valley fever virus, extending the range from Senegal to Madagasgar. Rift Valley fever antibody was shown to persist 49 years infection; the antibody was still in high titer. A new technique to conduct hybridization of ssRNA was developed as a modification of the Southern blot. Monoclonal antibody to Rift Valley fever virus established that there are at least 6 surface-reactive sites, 4 of these involved in the neutralization reaction. The ELISA was used effectively to screen Rift Valley fever vaccinees for antiboby. Both the IgM and IgC ELISA were developed for Rift Valley fever. The ELISA was also adapted to Crimean-Congo hemorrhagic fever virus for antibody detection. Fifty-two new acquisitions, including Japanese encephalitis, dengue yellow fever, phlebotomus fever viruses, and Ross River, were lyophilized and are available for investigators on request. Keywords: Viruses; Hemorrhagic fever viruses.

Descriptors :   *ARBOVIRUSES, *SEROLOGY, ANTIBODIES, CLONES, DENGUE, DETECTION, GLOBAL, GREECE, HEMORRHAGIC FEVERS, INFECTIOUS DISEASES, JAPANESE ENCEPHALITIS, NEUTRALIZATION, RESPONSE, RIFT VALLEY FEVER, RIFT VALLEY FEVER VIRUS, RIVERS, SANDFLY FEVER VIRUS INFECTION, SUDAN, VIRUSES, YELLOW FEVER, GROUP B ARBOVIRUSES, ARTHROPOD BORNE DISEASES, RIBONUCLEIC ACIDS, TAXONOMY, IMMUNOASSAY, ENZYMES, GEOGRAPHICAL DISTRIBUTION, TECHNICAL INFORMATION CENTERS

Subject Categories : Microbiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE