Accession Number : ADA181474

Title :   Amine Neurotransmitter Regulation of Long-Term Synaptic Plasticity in Hippocampus.

Descriptive Note : Annual rept. 1 Apr 86-31 Mar 87,

Corporate Author : BAYLOR COLL OF MEDICINE HOUSTON TX

Personal Author(s) : Johnston,Daniel

PDF Url : ADA181474

Report Date : 27 Apr 1987

Pagination or Media Count : 18

Abstract : The overall goal of this research project is to investigate the mechanisms of long-term synaptic potentiation (LTP) in hippocampus, with particular emphasis on the modulation of LTP by amine neurotransmitters. It was shown previously that norepinephrine (NE) enhances the magnitude, duration, and probability of induction of LTP at mossy fiber synapses. During the second year of this grant, intracellular recording techniques were utilized to explore various cellular hypotheses associated with this NE modulation of LTP. It was shown that the injection of cyclic AMP (cAMP) into the postsynaptic neuron could mimic the action of NE in enhancing LTP. This very important series of experiments has led to the current working hypothesis that NE modulates LTP by increasing cAMP in the postsynaptic neuron. Additional experiments, which explored possible membrane mechanisms associated with the action of NE on LTP, indicated that NE, through beta-adrenoceptors and cAMP, increases the activity of at least one type of voltage-dependent calcium channel. Other work associated with this grant involved the development of a preparation of mossy fiber synaptosomes for electrophysiological analysis and computer models for simulation of neural networks comprised of realistically represented hippocampal neurons.

Descriptors :   *SYNAPSE, *HIPPOCAMPUS, *NEUROCHEMICAL TRANSMISSION, COMPUTERIZED SIMULATION, FIBERS, CELLS(BIOLOGY), RECORDING SYSTEMS, LONG RANGE(TIME), MODULATION, ELECTROPHYSIOLOGY, NERVE CELLS, ADENOSINE PHOSPHATES, CYCLIC COMPOUNDS, INJECTION, PLASTIC PROPERTIES, MEMBRANES, LEVARTERENOL, CALCIUM, CHEMORECEPTORS, SIMULATION

Subject Categories : Biochemistry
      Anatomy and Physiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE