Accession Number : ADA181652

Title :   Human Immune Responses to Dengue Viruses.

Descriptive Note : Annual rept. Aug 85-Jun 86,

Corporate Author : MASSACHUSETTS UNIV MEDICAL CENTER WORCESTER MA

Personal Author(s) : Ennis,Francis A

PDF Url : ADA181652

Report Date : 01 Jul 1986

Pagination or Media Count : 23

Abstract : We have analyzed the interaction between the peripheral blood lymphocytes (PBL) of non-immune donors and dengue virus-infected cells, which results in interferon (IFN) production. Autologous monocytes or the Epstein-Barr virus transformed B lymyphoblastoid cell line (Raji cells) infected with dengue virus were used as IFN inducer cells. PBL produced IFN when cultured with dengue virus-infected cells. IFN was detected as early as 2 to 4 hours after exposure of PBL to dengue virus-infected cells. Dengue virus-infected cells treated with glutaraldehyde or paraformaldehyde, which produced no infectious dengue virus, also induced IFN. These results indicate that BPL produced IFN in response to dengue virus-infected cells and that the production of IFN by PBL is due to stimulation of PBL by dengue-virus-infected cells. The ability of dengue-infected cells to induce IFN correlated with the appearance of dengue antigens in infected cells. Characterization of IFN-producing PBL with monoclonal antibodies demonstrated that the IFN-producing cells were heterogeneous.

Descriptors :   *DENGUE, *IMMUNOLOGY, IMMUNOCHEMISTRY, ANTIGENS, CELLS, CELLS(BIOLOGY), CLONES, DONORS(MEDICINE), GROUP B ARBOVIRUSES, HUMANS, BLOOD, ANTIBODIES, INFECTIOUS DISEASES, INTERFERON, LYMPHOCYTES, PRODUCTION, STIMULATION(GENERAL), VIRUSES, STIMULATION(PHYSIOLOGY), BIOCHEMISTRY, LYSIS

Subject Categories : Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE