Accession Number : ADA182169

Title :   Dermal Influence on Epidermal Resurfacing during the Repair of Split Thickness Wounds.

Descriptive Note : Annual rept. 16 May 84-31 Mar 86, Final rept. 15 May 82-31 Mar 86.

Corporate Author : PITTSBURGH UNIV PA

Personal Author(s) : Hebda, Patrica A ; Eaglstein, William H

PDF Url : ADA182169

Report Date : Jun 1986

Pagination or Media Count : 103

Abstract : The purpose of this study was evaluate dermal-epidermal interrelationships during wound repair by assessing the effect on wound healing factors derived from platelets. Two methods were used to approach the problem: 1) an in vitro epithelization model in which small sections of partial-thickness pig skin (explants) were grown in medium containing fetal bovine serum and/or a platelet homogenate or purified-thickness platelet-derived factors,a nd eipidermal cell outgrowth from the explants was measured; 2) a wound healing animal model in which partial-thickness on epidermal cells. Our conclusions are that platelet components stimulate epidermal cell viability and outgrowth in explant cultures. We recommend further exploration of these factors. The factors should be purified from platelets, compared with known growth factors, and evaluated first in vitro with epidermal explant and cell culture systems. Active factors should be tested alone and in combination in animal would healing trails. Finally, an effective treatment should be evaluated in a clincal study with human subjects for effects on acute wounds, burn wounds and chronic ulcers.

Descriptors :   *SKIN(ANATOMY), *WOUNDS AND INJURIES, *HEALING, ANIMALS, REPAIR, BURNS(INJURIES), CELLS(BIOLOGY), BLOOD SERUM, BOVINES, FETUS, BLOOD PLATELETS, CULTURES(BIOLOGY), ULCERS, IN VITRO ANALYSIS, GROWTH(GENERAL), PURIFICATION, GROWTH(PHYSIOLOGY), SPLITTING

Subject Categories : Anatomy and Physiology
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE