Accession Number : ADA182270

Title :   Variations in Western Blot Banding Patterns of Human T-Cell Lymphotropic Virus Type III/Lymphadenopathy-Associated Virus,

Corporate Author : WALTER REED ARMY INST OF RESEARCH WASHINGTON DC

Personal Author(s) : Burke, Donald S. ; Redfield, Robert R. ; Putman, Pamela ; Alexander, Steve S.

Report Date : JAN 1987

Pagination or Media Count : 5

Abstract : Serum samples from 27 patients infected with human T -cell lymphotropic virus type III (14 with acquired immune deficiency syndrome AIDS and 13 with AIDS-related complex) were examined for antibodies to viral poteins by the Western blot method and with four different commercial solid-phase enzyme-linked immunosorbent assays (ELISAs). Virus-specific bands on blots at molecular masses 64, 55, 53, 41, 31, 24, and 17 kilodaltons were observed. Rank collelation matrices were calculated to relate the intensity of viral bands, stage of illness, and ELISA kit optical densities (ODs). Groups of bands tended to covary in intensity: p17, p24, and p55 (gang gene products); p53 and p64 (pol gene products); and p31 (pol/endonuclease gene product ) and p41 (env gene product). Blots of sera from AIDS-related complex patients usually showed strong activity against all viral poteins, while those of sera from AIDS patients characteristically showed strong reactivity only at the pol/endonuclease and env bands. For one ELISA kit (Abbott Laboratories, North Chicage, Ill.), ODs correlated well with the env and pol band intensity scores, while ELISA ODs with other kit (from Litton Industries, Sunnyvale, Calif; Electro-Nucleonics, Inc., Fairfeild, N.J.; and E.I. du Pont de Nemours & Co., Inc., Wilmington, Del.) correlated closely with gag band intensity scores. We concluded that human T-cell lymphotopic virus type III Western blot patterns are determined by (i) viral protein processing pathways and (ii) the stage of illness of the patient and may reflect (iii) the ELISA method used for serum screening. Reprints.

Descriptors :   *VIRUSES, *IMMUNOASSAY, *IMMUNOSUPPRESSION, PATIENTS, ENZYMES, GENES, SIGNS AND SYMPTOMS, ANTIBODIES, DENSITY, KITS, OPTICAL PROPERTIES, BANDS(STRIPS), PHOTOMETRY, INTENSITY, REPRINTS, BLOOD SERUM, REACTIVITIES, SAMPLING, PROTEINS.

Subject Categories : Medicine and Medical Research
      Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE