Accession Number : ADA182564

Title :   Regulation of Brain Neuropeptide Secretion by Lymphokines.

Descriptive Note : Annual rept. 1 Jul 86-30 Jun 87,

Corporate Author : NEW ENGLAND MEDICAL CENTER HOSPITAL BOSTON MASS

Personal Author(s) : Reichlin,Seymour ; Scarborough,David ; Dinarello,Charles ; Meir,James

PDF Url : ADA182564

Report Date : 01 Jul 1987

Pagination or Media Count : 3

Abstract : Preliminary experiments outlined in the original application suggested that supernatants from mononuclear cell cultures stimulated with staphylococcal antigens or IL-2 could release SRIF from dispersed cortical neurons. Such activated supernatants are known to contain many cytokines, including interleukins-1 (IL-1). We elected to begin the study of this release activity by examining the effects of IL-1 and IL-2 in more detail, using pure products of recombinant DNA origin. Human recombinant interleukin-1-beta (rIL-1B) was tested in both cortical and diencephalic cells. Modest SRIF release activity was observed only at a concentration of 10- M. To investigate the effect of chronic IL-1 exposure, cultures were incubated for 48 hours with various concentrations of rIL-1B and the cells and media assayed for SRIF. Keywords: Somatostatin.

Descriptors :   *LYMPHOCYTES, *NERVES, *PEPTIDES, *SECRETION, RELEASE, STAPHYLOCOCCUS, ANTIGENS, DEOXYRIBONUCLEIC ACIDS, GENETIC ENGINEERING, PURITY, CULTURES(BIOLOGY)

Subject Categories : Biochemistry
      Anatomy and Physiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE