Accession Number : ADA182612

Title :   Studies of RBC Preservation In-Vivo in a Rabbit Model.

Descriptive Note : Final rept. 1 May 84-31 Jul 86,

Corporate Author : MASSACHUSETTS UNIV MEDICAL CENTER WORCESTER MA

Personal Author(s) : Szymanski,Irma O

PDF Url : ADA182612

Report Date : Mar 1987

Pagination or Media Count : 44

Abstract : During blood storage at 4C, a progressively larger fraction of RBC become nonviable. Upon transfusion, the nonviable RBC are cleared extravascularly, but the exact site of their removal is not known. The number of nonviable RBC in a unit of blood can be determined only by transfusion studies in vivo. Both single and double label methods have been used to quantitate the number of nonviable RBC, although there is controversy about the accuracy of the single label method. It is not known which changes in stored RBC cause their rapid removal from circulation. The third component of complement accumulates on the RBC membrane during storage at 4C. It is possible that the C3 bound to stored RBC plays a role in the storage lesion by facilitating the rapid removal of the nonviable RBC. We conducted transfusion studies in rabbits to evaluate these issues in RBC preservation. Since some of them are difficult to evaluate in human subjects, the use of rabbit model is valuable. Our data showed that senescent rabbit RBC were destroyed in bone marrow and spleen. We were unable to demonstrate any RBC destruction in liver. In contrast, over 75% of the stored nonviable RBC were destroyed in bone marrow, 16 - 21% in the liver and the remainder in the spleen. The liver removed the nonviable RBC rapidly whereas the destruction in the spleen and bone marrow took place over a longer period of time. It is possible that the phagocytosis in the liver proceeded by a different mechanism than in the spleen and bone marrow.

Descriptors :   *BLOOD PRESERVATION, *ERYTHROCYTES, DESTRUCTION, LIVER, RETICULOENDOTHELIAL SYSTEM, ANATOMICAL MODELS, RABBITS, BONE MARROW, HUMANS, TIME, IN VIVO ANALYSIS, REMOVAL, LABELED SUBSTANCES, NECROSIS, BLOOD STORAGE, BLOOD COUNTS, VIABILITY, COMPLEMENT(BIOLOGY), SPLEEN, BLOOD TRANSFUSION, BLOOD, LESIONS

Subject Categories : Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE