Accession Number : ADA182872

Title :   Etiology and Rapid Diagnosis of Human Viral Gastroenteritis.

Descriptive Note : Annual rept. 1 May 86-30 Apr 87,

Corporate Author : MASSACHUSETTS UNIV MEDICAL SCHOOL WORCESTER

Personal Author(s) : Blacklow,Neil R

PDF Url : ADA182872

Report Date : 01 May 1987

Pagination or Media Count : 24

Abstract : This project aims to develop means for the rapid diagnosis of etiologic agents of human viral gastroenteritis and to employ these means for an assessment of the epidemiological roles of these viruses in diarrheal disease. In addition, we are studying the immunological relationships of various gastroenteritis viruses to aid in classification and diagonsis of these agents. Efforts have concentrated on the development and utilization of immunoassays to detect etiologic agents, with the preparation and use of monoclonal antibody reagents where possible. We have prepared monoclonal antibodies specific for the enteric adenoviruses types 40 and 41, viruses thought to be of major importance in diarrhea. These antibodies have been characterized and used in an enzyme immunoassay (EIA) format to detect the enteric adenoviruses in 71 known positive diarrheal stool specimens with 95 to 98 per cent sensitivity and specificity. We have confirmed the reported in vitro cultivation of 4 of 5 serotypes of human astrovirus, which is another agent that has been associated with some cases of gastroenteritis. Collaborative epidemiological studies are being done with the United States military on the role of enteric adenoviruses and Norwalk virus in overseas populations groups, including troops on training maneuvers in which we have already shown Norwalk virus to cause outbreaks of diarrhea. We expect to expand our testing ability to include astroviruses and possibly calciviruses in future studies.

Descriptors :   *ETIOLOGY, *GASTROENTERITIS, *DIAGNOSIS(MEDICINE), ANTIBODIES, CULTURES(BIOLOGY), DIARRHEA, FECES, ENZYMES, IMMUNOASSAY, EPIDEMIOLOGY, VIRUSES, ADENOVIRUSES, ENTERIC BACTERIA, DISEASE VECTORS, HUMANS, IMMUNOLOGY, CLONES, UNITED STATES, CLASSIFICATION, IN VITRO ANALYSIS, MILITARY PERSONNEL, TRAINING, UTILIZATION

Subject Categories : Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE