Accession Number : ADA182948

Title :   Influence of Neuroendocrine Mediators on Phagocyte Function.

Descriptive Note : Annual rept. 1 Jul 86-30 Jun 87,

Corporate Author : WAYNE STATE UNIV DETROIT MI DEPT OF BIOLOGICAL SCIENCES

Personal Author(s) : Petty,Howard R

PDF Url : ADA182948

Report Date : 01 Jul 1987

Pagination or Media Count : 11

Abstract : This research program explores the influences of neuroendocrine mediators on macrophage functions. Specifically, we have found the met-enkephalin and substance P enhance antibody-dependent phagocytosis of sheep red blood cells while epinephrine depresses phagocytosis. Many experimental results have been observed for both the RAW264 macrophage cell line and peritoneal exudate macrophages. In the complex physiological environment phagocytic cell receive many simultaneous but distinct ligand-receptor signals. To more closely reflect this environment in vitro, we have examined the effects of simultaneous treatment with met-enkephalin and epinephrine on RAW264 macrophage phagocytosis and cytolysis. We have discovered that at their physiologically-relevant doses these two ligands on the amount of phagocytosis were equivalent to or less than that of epinephrien alone. Therefore, in vitro models more closely approximating in vivo neuroregulation of macrophage function demonstrate phagocytic inhibition. Keywords: Stress; Macrophage; Phagocytosis; Cytolysis; Opioid; Adrenergic.

Descriptors :   *PHAGOCYTES, *RETICULOENDOTHELIAL SYSTEM, *HORMONES, *IMMUNOCHEMISTRY, *NEUROCHEMISTRY, *ANTIBODIES, CYTOLOGY, EPINEPHRINE, IN VIVO ANALYSIS, EXUDATION, PERITONEUM, PHAGOCYTES, ERYTHROCYTES, SHEEP, IN VITRO ANALYSIS, PHAGOCYTES, STRESS(PHYSIOLOGY), OPIUM ALKALOIDS, IMMUNITY, PEPTIDES, LIGANDS

Subject Categories : Biochemistry
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE