Accession Number : ADA182953
Title : The Primary Structure of Acetylcholinesterase and Selective Antibodies for the Detection of Organophosphate Toxicity.
Descriptive Note : Annual rept. 30 Sep 84-30 Sep 85,
Corporate Author : CALIFORNIA UNIV SAN DIEGO LA JOLLA DIV OF PHARMACOLOGY
Personal Author(s) : Taylor,Palmer W
PDF Url : ADA182953
Report Date : 03 Dec 1985
Pagination or Media Count : 19
Abstract : The proposed work has been directed to determination of the structure of acetylcholinesterase. During the project period we have determined the primary structure of the Torpedo enzyme through amino acid sequencing and the isolation and sequencing of a c-DNA clone encoding for the 11S form of the enzyme. Peptides corresponding to the active center of the enzyme and a C-terminal region have been synthesized and antibodies are being raised for the purpose of detecting the phosphorylated enzyme and delineating functional regions of the molecule. Finally, progress has been made on the secondary structure of the enzyme. Keywords: Biochemistry.
Descriptors : *ACETYLCHOLINESTERASE, *ORGANOPHOSPHATES, *TOXICITY, *ANTIBODIES, *MOLECULAR STRUCTURE, BIOCHEMISTRY, ENZYMES, PEPTIDES, SECONDARY, AMINO ACIDS, ISOLATION, SYNTHESIS, SEQUENCES, DEOXYRIBONUCLEIC ACIDS, SHARKS
Subject Categories : Biochemistry
Genetic Engineering and Molecular Biology
Distribution Statement : APPROVED FOR PUBLIC RELEASE