Accession Number : ADA182953

Title :   The Primary Structure of Acetylcholinesterase and Selective Antibodies for the Detection of Organophosphate Toxicity.

Descriptive Note : Annual rept. 30 Sep 84-30 Sep 85,

Corporate Author : CALIFORNIA UNIV SAN DIEGO LA JOLLA DIV OF PHARMACOLOGY

Personal Author(s) : Taylor,Palmer W

PDF Url : ADA182953

Report Date : 03 Dec 1985

Pagination or Media Count : 19

Abstract : The proposed work has been directed to determination of the structure of acetylcholinesterase. During the project period we have determined the primary structure of the Torpedo enzyme through amino acid sequencing and the isolation and sequencing of a c-DNA clone encoding for the 11S form of the enzyme. Peptides corresponding to the active center of the enzyme and a C-terminal region have been synthesized and antibodies are being raised for the purpose of detecting the phosphorylated enzyme and delineating functional regions of the molecule. Finally, progress has been made on the secondary structure of the enzyme. Keywords: Biochemistry.

Descriptors :   *ACETYLCHOLINESTERASE, *ORGANOPHOSPHATES, *TOXICITY, *ANTIBODIES, *MOLECULAR STRUCTURE, BIOCHEMISTRY, ENZYMES, PEPTIDES, SECONDARY, AMINO ACIDS, ISOLATION, SYNTHESIS, SEQUENCES, DEOXYRIBONUCLEIC ACIDS, SHARKS

Subject Categories : Biochemistry
      Genetic Engineering and Molecular Biology
      Toxicology

Distribution Statement : APPROVED FOR PUBLIC RELEASE