Accession Number : ADA183321
Title : Evolution of Phosphofructokinase.
Descriptive Note : Annual rept. 1 Aug 86-31 Jul 87,
Corporate Author : UNIVERSITY OF HEALTH SCIENCES/CHICAGO MEDICAL SCHOOL IL DEPT OF BIOLOGICAL CHEMISTRY AND STRUCTURE
Personal Author(s) : Kemp,Robert G
PDF Url : ADA183321
Report Date : 31 Jul 1987
Pagination or Media Count : 3
Abstract : Comparative sequence data suggest that eucaryotic phosphofructokinase (PFK) has evolved from a procaryotic precursor by gene duplication, fusion, and mutation of previous catalytic sites into new regulatory ligand binding sites. We are attempting to duplicate these processes by recombinant DNA technology. The E. coli pfk gene is being recloned into a convenient plasmid. Using a synthetic oligonucleotide that matches the mammalian link region, two E. coli pfk genes will be joined. The properties of the expressed duplex enzyme will be analyzed. Utilizing site-directed mutagenesis, conversion of one of the two catalytic sites to a regulatory site will be carried out. Keywords: Protein evolution, Protein engineering.
Descriptors : *DEOXYRIBONUCLEIC ACIDS, *GENETIC ENGINEERING, *PHOSPHORUS TRANSFERASES, *CLONES, SITES, CHROMOSOMES, EVOLUTION(GENERAL), PROTEINS, SEQUENCES, GENES, CATALYTIC CRACKING, ESCHERICHIA COLI, CATALYSIS, MUTATIONS
Subject Categories : Biochemistry
Genetic Engineering and Molecular Biology
Distribution Statement : APPROVED FOR PUBLIC RELEASE