Accession Number : ADA183804

Title :   Comparative Infectivity Determination of Candidate Live Dengue Virus Vaccine in Monkeys, Mosquitoes and Cell Cultures.

Descriptive Note : Final rept. 1 Jun 84-31 May 87,

Corporate Author : PUERTO RICO UNIV SAN JUAN

Personal Author(s) : Kraiselburd,Edmundo

PDF Url : ADA183804

Report Date : 27 Jul 1987

Pagination or Media Count : 21

Abstract : The following live, attenuated dengue virus vaccines strains were tested for their respective immunogonicity in rhesus monkeys: dengue-1 (TP79-56, code TSI-GSD216 and 45AZ-5, code No. TSI-GSD214; dengue 3 (CH53489, clone 24/28); and dengue-4 (341750 Carib). It was found that 417 MID50 (mosquito infectious dose 50) and 116 MID50 were required for seroconversion of 50% of the animals with the 45AZ5 and the TP79-56 vaccine, respectively. Upon challenge with the homologous wild type virus, dengue-1 immune animals showed a typical secondary antibody response. A dose of 2.9 MID50 (dengue-3 vaccine) and of 0.158 MID50 (parent dengue-3 virus) seroconverted 50% of the animals. Experimental dat showed that the DEN-4 vaccine was highly attenuated: a dose of 60 MID50 of the parent, wild type dengue-4 virus (341750 Carib) was required to elicit seroconversion of 50% of the experimentl animals. The dengue-4 vaccine may be suitable for trials with human volunteers.

Descriptors :   *DENGUE, *GROUP B ARBOVIRUSES, *LIVE VACCINES, *IMMUNITY, ANTIBODIES, CELLS(BIOLOGY), CULTURES(BIOLOGY), DENGUE, GROUP B ARBOVIRUSES, HUMANS, RESPONSE(BIOLOGY), RHESUS MONKEYS, STRAINS(BIOLOGY), VOLUNTEERS, WILDLIFE, STRAINS(BIOLOGY), IMMUNIZATION

Subject Categories : Pharmacology
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE