Accession Number : ADA183806

Title :   Computer Image Analysis of Histochemically-Labeled Acetylcholinesterase.

Descriptive Note : Annual rept. 30 Sep 83-29 Sep 84,

Corporate Author : TEXAS UNIV MEDICAL BRANCH AT GALVESTON

Personal Author(s) : Hillman,Gilbert R

PDF Url : ADA183806

Report Date : 30 Nov 1984

Pagination or Media Count : 32

Abstract : The purpose of the study is to explore the use of computer image analysis on conjunction with histochemical techniques to describe the distribution of acetylcholinesterase (AChE) activity in nervous and muscular tissue in rats treated with organophosphates (OPs). The objective of the first year of work on this remaining 2 years. We began by adopting a version of the AChE staining method as modified by Hanker, which consistent with the optical properties of our video system. We wrote computer programs for provide a numeric quantity which represents the degree of staining in a tissue section. The staining was calibrated by experiments in which brain tissue was homogenized and AChE measured by biochemical methods. Other experiments examined the effects of a varying the incubation period for staining, and of inhibiting AChE by addition of carbamates or OPs to the staining reaction. Limited experiments using in vivo inhibition of AChE in rats by diisopropylfluorophosphonate (DFP) showed strong inhibition in stained brain section. The results of these studies indicate that the determination and mapping of AChE by quantitative histochemistry is feasible and reliable.

Descriptors :   *COMPUTER APPLICATIONS, *IMAGE PROCESSING, *ACETYLCHOLINESTERASE, *HISTOCHEMISTRY, *HISTOLOGICAL TECHNIQUES, *BIOLOGICAL STAINS, BIOCHEMISTRY, BRAIN, CALIBRATION, CARBAMATES, COMPUTER APPLICATIONS, COMPUTER PROGRAMS, FLUORINE COMPOUNDS, INCUBATION, LABELED SUBSTANCES, OPTICAL PROPERTIES, ORGANOPHOSPHATES, PHOSPHONATES, RATS, TISSUES(BIOLOGY), VIDEO SIGNALS, CHOLINESTERASE INHIBITORS, POISONING

Subject Categories : Biochemistry
      Toxicology

Distribution Statement : APPROVED FOR PUBLIC RELEASE