Accession Number : ADA184048

Title :   Bimane Derivatives as Fluorescent Probes for Biological Macromolecules.

Descriptive Note : Final rept. Nov 83-Dec 86,

Corporate Author : TEL-AVIV UNIV (ISRAEL) DEPT OF CHEMISTRY

Personal Author(s) : Kosower,Edward M.

Report Date : 18 APR 1987

Pagination or Media Count : 28

Abstract : Syn-9, 10-Dixoabimanes (1,5-diazabicyclo3.3.0octa-3,6-diene-2,8-diones) are photostable, highly fluorescent compounds which can be attached to biological macromolecules as fluorescent labels. Syntheses of the important new intermediates for labeling agents, syn-tricyclic bimanes, RuCn (R=CH3 or C1) and n= 0.1,2,3 have been developed. The conversion of the compounds with n=2 into a diene has been accomplished, successfully raising the adsorption coefficient. However, interesting and surprising physical behavior conspired to drastically diminish the fluorescence quantum yield. The discovery of several routes to unsymmetrically substituted bimanes prepared by nucleophilic displacement on bromoalkyl derivatives has revealed new classes of labeling agents for macromolecules. These include a cross-linking agent.

Descriptors :   *FLUORESCENCE, *BIOASSAY, *HETEROCYCLIC COMPOUNDS, *SYNTHESIS(CHEMISTRY), ADSORPTION, COEFFICIENTS, VITAMIN B COMPLEX, CONVERSION, QUANTUM THEORY, YIELD, LABELS, MACROMOLECULES, DISPLACEMENT, BIOMOLECULES, RADIOPROTECTIVE AGENTS, CROSSLINKING(CHEMISTRY), PROBES, MEMBRANES, PROTEINS, PHYSICAL PROPERTIES

Subject Categories : Organic Chemistry
      Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE