Accession Number : ADA184180

Title :   Monoclonal Antibodies that Recognize Proteins Unique to Somatic Embryos of Daucus carota.

Descriptive Note : Summary rept. 1 Oct 84-30 Sep 85,

Corporate Author : CALIFORNIA UNIV OAKLAND NAVAL BIOSCIENCES LAB

Personal Author(s) : Smith,J A ; Choi,J ; Krauss,M ; Karu,Alexander E ; Sung,Z R

PDF Url : ADA184180

Report Date : Jan 1985

Pagination or Media Count : 10

Abstract : We have developed a panel of monoclonal antibodies (Mabs) to several antigens of Daucus carota somatic embryos. We prepared over 2400 hybridoma cultures from splenocytes of Balb/c mice immunized with lyophilized somatic embryo cells. The MAbs were screened on embryo, callus cell, and chloroplast extracts using an automated sampling system and computer-assisted analyses. Most of the MAbs reacted with antigens common to embryo and callus cells, nut MAbs from al least 5 hybridoma lines recognized single antigens unique to the somatic embryos. Other lines produced antibodies to chloroplast antigens, including the light-harvesting chlorophyll protein (LHCP).

Descriptors :   *ANTIBODIES, *PLANT TISSUE, ANTIGENS, EMBRYOS, COMPUTER AIDED DIAGNOSIS, CLONES, MICE, PROTEINS, CHLOROPLASTS, CHLOROPHYLLS, RECOGNITION, CELLS(BIOLOGY), SAMPLING, AUTOMATION, IMMUNOASSAY, ENZYMES

Subject Categories : Genetic Engineering and Molecular Biology

Distribution Statement : APPROVED FOR PUBLIC RELEASE