Accession Number : ADA184366

Title :   Stabilization of Z-RNA under Physiological Conditions and Recognition by Anti-Z DNA Antibodies.

Descriptive Note : Summary rept. 1 Oct 86-30 Sep 87,

Corporate Author : CALIFORNIA UNIV OAKLAND NAVAL BIOSCIENCES LAB

Personal Author(s) : Hardin,Charles C ; Zarling,David A ; Puglisi,Joseph D ; Trulson,Mark O ; Davis,Peter W

PDF Url : ADA184366

Report Date : Jan 1987

Pagination or Media Count : 38

Abstract : Radioimmunoassay and nitrocellulose filter-binding competition experiments were performed to determine the extent of recognition of Bromine-poly(r(C-G) by anti-Z-DNA antibodies. The polyclonal rabbit anti-Br-poly(d(C-G)igG preparations T4 and Z6 specifically recognize the Z-form of polyR(C-G, although the binding affinities are 10-to 100fold lower for Z-RNA than for various forms of Z-DNA. Competition RIA experiments verify the presence of a Z-DNA like determinant in left handed Br poly R(C-G) at physiological Sodium Chloride concentration. Phosphate buffer or high ionic strength conditions inhibit recognition of Z-RNA by anti Z-DNA IgGs, consistent with recognition of a phosphodiester backbone determinant. In summary, these spectroscopic and immunochemical studies demonstrate that under conditions of conformational stress (i.e. containing brominated nucleosides) left-handed Z-RNA is stable and is specifically recognized by proteins at physiological temperature and ionic strength.

Descriptors :   *RIBONUCLEIC ACIDS, *DEOXYRIBONUCLEIC ACIDS, *ANTIBODIES, *RADIOIMMUNOASSAY, IMMUNOCHEMISTRY, NITROCELLULOSE, FILTERS, BONDING, BROMINATION, IMMUNOGLOBULINS, RECOGNITION, STABILITY, GUANINE, CYTOSINE, NUCLEOSIDES, SPECTROSCOPY

Subject Categories : Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE