Accession Number : ADA184832

Title :   The Nitrogenase in a Methanogenic Archaebacterium and Its Regulation.

Descriptive Note : Annual rept. Sep 86-Aug 87,

Corporate Author : NEW YORK STATE COLL OF AGRICULTURE AND LIFE SCIENCES ITHACA DEPT OF MICROBIOL OGY

Personal Author(s) : Zinder, Stephen H

PDF Url : ADA184832

Report Date : 31 Aug 1987

Pagination or Media Count : 6

Abstract : The objectives of this project are to gain an understanding of the process of nitrogen fixation in the methanogenic archaebacterium, Methanosarcina barkeri strain 227. Initial studies centered on the growth physiology of M. barkeri under diazotrophic conditions. We have also demonstrated that crude extracts of diazotrophic M. Barkeri reduce acetylene at a very low rate, but apparently reduce dinitrogen to ammonia at a 20-fold higher rate per electron. Among the objectives for the coming year are further characterization of reactions carried out by the nitrogenase in crude extracts, and purification of the nitrogenase enzymes.

Descriptors :   *ANAEROBIC BACTERIA, *NITROGEN, *OXIDOREDUCTASES, *ASSIMILATION, REDUCTION(CHEMISTRY), BIOCHEMISTRY, ACETYLENE, METHANE, BIOSYNTHESIS

Subject Categories : Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE