Accession Number : ADA184884

Title :   The Microbial Degradation of TCE (Trichloroethylene).

Descriptive Note : Final rept. 30 Apr 83-31 Jul 86,

Corporate Author : AIR FORCE ENGINEERING AND SERVICES CENTER TYNDALL AFB FL ENGINEERING AND SERV ICES LAB

Personal Author(s) : Wolfram, J H ; Case, J T ; Rogers, R D

PDF Url : ADA184884

Report Date : Apr 1987

Pagination or Media Count : 55

Abstract : The objective of this research was to characterize naturally occurring microorganisms capable of degrading trichloroethylene (TCE). Microorganisms tolerant to TCE were isolated from an aeration tower at Wurtsmith AFB MI and from industrial sludges at Hill AFB UT. Isolates from the Wurtsmith samples exhibited the highest density of cells with TCE as the only apparent organic carbon source and were selected as the inoculum for degradation studies. However, the capability of selected microorganisms to degrade TCE was not substantiated in both gas chromatography analyses and samples inoculated in culture bottles. Experiments using radiolabeled carbon-14 to study assimilation suggest that TCE is metabolized into a polar form by the microorganism. Subsequent cultures grown in radiolabeled TCE did not duplicate the previous results possible because of contamination, selection of variants or loss of culture. The organism responsible or the apparent TCE degradation may belong in the genus Citrobacter.

Descriptors :   *ENTEROBACTERIACEAE, *TRICHLOROETHYLENE, *BIODETERIORATION, CULTURES(BIOLOGY), LABELED SUBSTANCES, GAS CHROMATOGRAPHY, SLUDGE, ASSIMILATION, METABOLISM

Subject Categories : Biochemistry
      Microbiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE