Accession Number : ADA185617

Title :   Red Cell Storage Studies.

Descriptive Note : Final rept. 16 Jun 83-31 Mar 86,

Corporate Author : CALIFORNIA UNIV SAN FRANCISCO

Personal Author(s) : Shohet, Stephen B

PDF Url : ADA185617

Report Date : 15 Apr 1987

Pagination or Media Count : 19

Abstract : Efforts were focused in 5 major areas: we established a strong correlation between in vitro red cell deformability measurements an in vivo red cell survival measurements. We found that a red cell deformability defect could be induced by an increase in membrane instability secondary to a calcium: calmodulin interaction which may occur in stored cells. In collaborative studies, we found that appreciable membrane vesiculation occurs during in vitro storage under blood bank conditions. We studied the antibody-mediated accumulation of gamma-globulin on red cells and some of the mechanism of this process both with regards to the so-called 'senescent' antigen, and the anti-Gal antigen. We re-examined the assumption that cell age is directly related to cell density, and concluded that a simple correlation of cell age is not tenable. Keywords: Blood storage; Membranes(Biology); Blood cells.

Descriptors :   *BLOOD STORAGE, *ERYTHROCYTES, AGING(PHYSIOLOGY), BLOOD BANKS, BLOOD CELLS, CALCIUM, CELLS, CELLS(BIOLOGY), CORRELATION, DENSITY, IN VITRO ANALYSIS, MEMBRANES, STABILITY, STORAGE, SURVIVABILITY, MEMBRANES(BIOLOGY), BLOOD PRESERVATION, IMMUNOGLOBULINS, DEFORMATION

Subject Categories : Medical Facilities, Equipment and Supplies
      Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE