Accession Number : ADA185991

Title :   Human Immune Response to Dengue Infections.

Descriptive Note : Annual rept. Jul 86-Jun 87,

Corporate Author : MASSACHUSETTS UNIV MEDICAL CENTER WORCESTER MA

Personal Author(s) : Ennis, Francis A

PDF Url : ADA185991

Report Date : 30 Jul 1987

Pagination or Media Count : 16

Abstract : We have analyzed human T cell responses to dengue virus. Peripheral blood lymphocytes from dengue-immune Thai donors and two American donors who were infected with low passaged CH53489 strain of dengue 3 virus were used. Sonicated dengue-infected Vero cells induced significant proliferative responses of PBL from non-immune donors, but did not induce specific proliferative responses of PBL from non-immune donors. IFN was detected in the culture fluid of denque-immune PBL stimulated with denque antigen. A short term-cultured line was established using PBL from one American donor. Phenotypic analysis showed that they are T cell with CD4 phenotype (CD3+, CD4+, CD8-, CD16-, CD20-). They responded to denque 3 antigen, and produced IFN. Then, 14 denque-specific T cell clones/lines were established by a limiting dilution method using PBL from same American donor. All clones/lines responded to denque 3 antigen, but none of them responded to control antigen. Four lines were examined for denque-immune donors contain denque-specific T cells with CD4 phenotype, and that these CD4+ cells produce IFN in response to denque antigen. Keywords: Antigen antibody responses.

Descriptors :   *DENGUE, *IMMUNITY, ANTIBODIES, ANTIGENS, BLOOD, CONTROL, DILUTION, DONORS(MEDICINE), GROUP B ARBOVIRUSES, HUMANS, INFECTIOUS DISEASES, LIMITATIONS, LYMPHOCYTES, RESPONSE(BIOLOGY), ANTIGEN ANTIBODY REACTIONS, INTERFERON

Subject Categories : Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE