Accession Number : ADA186824

Title :   Purified Mojave Toxin Interacts with CA++Mg++ ATPase in Rat Synaptic Membranes.

Descriptive Note : Contractor rept. Jan-Apr 86,

Corporate Author : TEXAS UNIV AT SAN ANTONIO DIV OF LIFE SCIENCES

Personal Author(s) : Chambers, James P ; Wayner, Matthew J ; Valdes, James J

PDF Url : ADA186824

Report Date : Aug 1987

Pagination or Media Count : 17

Abstract : Calcium mediated channels produce changes in intracellular calcium concentration in neural, skeletal, and cardiac tissues. The Ca Mg ATPase is the enzyme responsible for maintaining the Ca gradient across the membrane. Studies of these Ca dependent membrane proteins have been limited due to the scarcity of naturally occuring toxins with which to probe the systems. In this report, we describe the actions of Mojave toxin, an acidic toxin isolated from the venom of Crotalus scutulatus scutulatus, on the Ca Mg ATPase and its associated dihydropyridine receptor sites. Mojave toxin induced an increase in rat brain synaptic membrane Ca++Mg++ ATPase activity and inhibited dihydropyridine binding. These data suggest that Mojave toxin may be a useful probe of the Ca channel complex.

Descriptors :   *SYNAPSE, *TOXINS AND ANTITOXINS, *HYDROLASES, *MEMBRANES(BIOLOGY), ACIDS, BRAIN, CALCIUM, CHANNELS, ENZYMES, HEART, PROTEINS, RATS, TISSUES(BIOLOGY), ADENOSINE PHOSPHATES, CALCIUM, MAGNESIUM, CATIONS, NEUROCHEMICAL TRANSMISSION, NERVES, PYRIDINES, VENOMS

Subject Categories : Toxicology
      Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE