Accession Number : ADA188311

Title :   Receptor Proteins in Vertebrate Olfaction.

Descriptive Note : Final rept. 1 Jun 83-31 May 87,

Corporate Author : VIRGINIA COMMONWEALTH UNIV RICHMOND

Personal Author(s) : Price, Steven

PDF Url : ADA188311

Report Date : 15 Oct 1987

Pagination or Media Count : 7

Abstract : Prior to beginning this project we had developed two hypothesis: (1) that receptor protein in olfactory receptor cells comprise a class of molecules of related structure with a region common to all and a region specific for a particular odorant class; (2) that interaction with receptor proteins is only one of several mechanisms by which olfactory neurons are stimulated by odorants. This project was basically an attempt to test these hypothesis. Proteins with affinity for compounds related to the odorants, anisole and benzaldehyde, were isolated from extracts of dog olfactory epithelium by affinity chromatography. Sera from rabbits immunized with each were general inhibitors of mouse olfactory tissue to odorant stimulation. Monoclonal antibodies were prepared against each protein and tested for ability to alter responses to mouse olfactory epithelium to odorant stimulation. The data are consistent with the hypothesis that olfactory receptor proteins are members of a structurally related class of which the anisole and benzaldehyde binding proteins are members. None of the antibodies reduced the magnitude of the response must be mediated by some pathway other than initiation by binding of the odorant to the receptor protein. This is consistent with the hypothesis that there are multiple mechanisms by which odorants stimulate olfactory neurons, interaction with receptor proteins being one of them.

Descriptors :   *PROTEINS, *SMELL, *OLFACTORY NERVE, ALDEHYDES, ANISOLE, ANTIBODIES, BENZYL RADICALS, CHROMATOGRAPHY, CLONES, DOGS, EPITHELIUM, INHIBITORS, MICE, MOLECULES, NERVE CELLS, ODORS, RABBITS, STIMULATION(PHYSIOLOGY), MOLECULE MOLECULE INTERACTIONS

Subject Categories : Anatomy and Physiology
      Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE