Accession Number : ADA188570

Title :   Structure and Expression of Genes for Flavivirus Immunogens.

Descriptive Note : Annual rept. 1 Sep 84-31 Aug 85,

Corporate Author : MASSACHUSETTS UNIV AMHERST DEPT OF BIOCHEMISTRY

Personal Author(s) : Fournier, Maurille J ; Mason, Thomas L

PDF Url : ADA188570

Report Date : 01 Sep 1985

Pagination or Media Count : 47

Abstract : Banks of cloned cDNA and specific antibodies are being used to characterize the structure and expression of the genomes of two flaviviruses, the Japanese encephalitis virus (JEV) and the dengue-type 1 virus (DEN-1). Major aims of the program are to gain insight into the mechanism of viral replication, to characterize the immunopathological response of the mammalian host and to identify peptide immunogens that could be appropriate for use as an antiviral vaccine and for viral diagnosis. It will be possible to map with high resolution individual submolecular domains that define neutralizing and protective epitopes. Immunogens encompassing these elements are obvious candidates for use in vaccine development. The results also demonstrate that virus-specific and conserved epitopes defined at the virion level are also evident at the protein molecular level. This situation offers the promise of developing both specific and broadly cross-reactive antigens and antibodies for use as productive immunogens and viral diagnosis.

Descriptors :   *GENES, *GROUP B ARBOVIRUSES, *IMMUNOGENS, *VACCINES, *DIAGNOSIS(MEDICINE), *DENGUE, ANTIBODIES, HIGH RESOLUTION, JAPANESE ENCEPHALITIS VIRUSES, MAMMALS, PEPTIDES, PROTEINS, CLONES, DEOXYRIBONUCLEIC ACIDS, MOLECULAR STRUCTURE

Subject Categories : Pharmacology
      Microbiology
      Genetic Engineering and Molecular Biology

Distribution Statement : APPROVED FOR PUBLIC RELEASE