Accession Number : ADA188579

Title :   Cellular Response to Exogenous Electromagnetic Fields.

Descriptive Note : Annual rept. Oct 86-Sep 87,

Corporate Author : COLUMBIA UNIV NEW YORK DEPT OF PATHOLOGY

Personal Author(s) : Goodman, Reba ; Henderson, Ann S.

Report Date : 21 SEP 1987

Pagination or Media Count : 5

Abstract : Our research has determined that extremely low frequency (ELF) (100 Hz) electromagnetic (EM) fields induce alterations in transcription and translation in dipteran (Drosophila, Sciara) and human (HL60, IB4) cells. Transcriptional autoradiography and endoreplicated chromosomes, as well as analyses of sucrose density gradient profiles, shows increased uptake of 3H uridine into RNA following exposure of cells to sinusoidal and asymmetric quasi-rectangular signals for time periods up to 60 minutes. Biochemical analyses (sucrose density gradients, oligo (d) T columns) have demonstrated that the RNA affected by signal stimulation is in the messenger RNA size class. Quantitative and qualitative alterations also occur in polypeptide synthesis following EM field stimulation. Major differences are noted from control among all sets analyzed with respect to new and augmented, as well as suppressed and deleted polypeptides. Using hybridization analysis, there is preliminary evidence that cell specific transcripts are augmented. Keywords: Polypeptides, Genes, Waveform characteristics, Cells(biology).

Descriptors :   *ELECTROMAGNETIC FIELDS, *GENES, *PEPTIDES, *STRESS(PHYSIOLOGY), *CELLS(BIOLOGY), AUTORADIOGRAPHY, BIOCHEMISTRY, CHROMOSOMES, DENSITY, DROSOPHILA, EXTREMELY LOW FREQUENCY, GRADIENTS, HYBRIDIZATION, POLYMERS, PROFILES, SIGNALS, SUCROSE, TIME INTERVALS, WAVEFORMS, RIBONUCLEIC ACIDS, STIMULATION(PHYSIOLOGY), HUMANS.

Subject Categories : Stress Physiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE