Accession Number : ADA188787

Title :   Contributions of Interleukin-1 to Nonspecific Antibacterial Resistance.

Descriptive Note : Annual rept. 27 Mar-25 Nov 87,

Corporate Author : WISCONSIN UNIV-MADISON SCHOOL OF VETERINARY MEDICINE

Personal Author(s) : Czuprynski, Charles J.

PDF Url : ADA188787

Report Date : 12 NOV 1987

Pagination or Media Count : 2

Abstract : The purpose of this project was to examine the influence of parenteral administration of interleukin-1, a cytokine with diverse biological activities, on antibacterial resistance in a laboratory rodent model. We first documented that intraperitoneal injection of minute quantities of interleukin-1 resulted in a rapid influx of inflammatory neutrophils. Neutrophil accumulation did not result from contamination of the interleukin-1 with bacterial lipopolysaccharide, nor was it abrogated by treatment with indomethacin, an inhibitor of prostagladin synthesis. We also observed a small but significant increase in the number of inflammatory macrophages at later timepoints. We went on to show that prophylactic or concomitant administration of interleukin-1 (0.17 ug per mouse) significantly enhanced the resistance of recipient mice to a challenge infection with the facultative intracellular pathogen Listeria monocytogenes. Protection was not caused by contaminating bacterial lipopolysaccharide. Interleukin-1 mediated protection was associated with a rapid burst of serum colony--stimulating activity. Current experiments are comparing the separate and combined effects of interleukin-1 and other cytokines on antibacterial resistance. We also will examine the influence of an in vivo and in vitro administration of interleukin-1 on leukocyte function. These observations suggest that pretreatment with interleukin-1 might be beneficial for those at increased risk of bacterial infection.

Descriptors :   *BACTERIAL DISEASES, *ANTIMICROBIAL AGENTS, *IMMUNOCHEMISTRY, CONTAMINATION, GERMICIDES, IN VITRO ANALYSIS, IN VIVO ANALYSIS, INFECTIOUS DISEASES, INFLAMMATION, INTRAMUSCULAR INJECTIONS, LEUKOCYTES, LIPOPOLYSACCHARIDES, MICE, MODELS, MUTAGENS, PARENTERAL INFUSIONS, PERITONEUM, PHAGOCYTES, POLYMORPHONUCLEAR NEUTROPHILS, RETICULOENDOTHELIAL SYSTEM, RISK, RODENTS, COLONIES(BIOLOGY), RESISTANCE(BIOLOGY).

Subject Categories : Pharmacology
      Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE