Accession Number : ADA189119

Title :   Mode of Action of Shigella Toxin: Effects on Ribosome Structure and Function.

Descriptive Note : Annual rept. 2 Aug 84-14 Sep 85,

Corporate Author : ALBANY MEDICAL COLL NY

Personal Author(s) : Obrig, Tom G.

PDF Url : ADA189119

Report Date : 01 OCT 1985

Pagination or Media Count : 25

Abstract : The goals of this research contract are to examine the primary action of Shigella dysenteriae 1 toxin (Shiga toxin). As an inhibitor of eukaryotic protein biosynthesis. Two major objectives of this investigation are designed to reveal Shiga toxin-induced changes in ribosome structure-function relationships. These objectives are 1) to explain, in biochemical terms, the manner by which Siga toxin enzymatically inactivates mammalian ribosomes and 2) to define the steps of protein biosynthesis which are specifically inhibited by the toxin as a result of ribosome modification. A comparison between Shiga toxin and similar fungus- and plant-derived toxins is to be made in regard to ribosome inactivation. Information obtained from these studies will be of value in describing the role of Shiga toxin in establishment of intestinal infections by the toxin-producing Shigella species. Delineated how Shiga toxin inhibits peptide elongation in reticulocyte protein synthesis. We have shown, that Shiga toxin does not inhibit peptidyl transferase, which catalyzes peptide bond formation.

Descriptors :   *RIBOSOMES, *SHIGELLA, *BACTERIAL TOXINS, *PROTEIN METABOLISM, *TOXICITY, BIOCHEMISTRY, BIOSYNTHESIS, ELONGATION, FUNGI, INACTIVATION, INFECTIOUS DISEASES, INTESTINES, MAMMALS, MODIFICATION, PEPTIDES, PROTEINS, REACTION KINETICS, GRAM NEGATIVE BACTERIA, TOXIC TOLERANCES.

Subject Categories : Toxicology
      Microbiology
      Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE