Accession Number : ADA189154
Title : Radiofrequency/Microwave Cell Absorption and Action Spectroscopy.
Descriptive Note : Annual rept. 1 Sep-31 Aug 86,
Corporate Author : MEDICAL COLL OF VIRGINIA RICHMOND DEPT OF PHYSIOLOGY AND BIOPHYSICS
Personal Author(s) : Cleary, Stephen F ; Liu, Li-Ming
PDF Url : ADA189154
Report Date : 10 Nov 1987
Pagination or Media Count : 13
Abstract : Research during the contract year has included the following: a) an in vitro tendon explant model system has been used to investigate the effects of weak ELF fields on fibroplasia and collagen synthesis. Maximum (33%) fibroplasia (relative to sham-exposed explants) was induced by 1Hz square wave pulsed fields at a time averaged current density of 7mA/sq.m. Under these conditions there were no effects on relative collagen synthesis. Lower or higher current densities had relatively less effects on fibroplasia. Maximal response occurred in explants oriented parallel rather than perpendicular to the E-field. At current densities of 14mA/sq. m, fibroplasia and collagen synthesis were suppressed but noncollagen protein synthesis was unaffected. A series of duplicate experiments was conducted to determine the effects of pulsed magnetic fields from Helmholtz coils on tendon explant fibroplasia and collagen synthesis. No effects were detected on either dependent variable. In order to relate pulsed external applied fields to cellular alterations quasi-steady solution of Laplace's equation was obtained and programmed for computation. This solution will be used to calculate induced plasmalemma, nuclear and organelle membrane potentials and karyoplasmic and cytoplasmic potentials in response to pulsed DC electric fields and CW and pulsed RF fields. Keywords: Electric fields, Magnetic fields, Tendon fibroplasia, Collagen synthesis, Protein synthesis, Laplace equation, Member induced potential, Phase transition liposomes.
Descriptors : *BIOSYNTHESIS, *COLLAGEN, *CYTOPLASM, *ELECTRIC FIELDS, *LAPLACE TRANSFORMATION, *MAGNETIC FIELDS, *PROTEINS, ABSORPTION, CELLS, DENSITY, DIRECT CURRENT, EQUATIONS, EXTERNAL, EXTREMELY LOW FREQUENCY, IN VITRO ANALYSIS, LOW DENSITY, LOW STRENGTH, MICROWAVES, MODELS, PHASE TRANSFORMATIONS, PULSES, RADIOFREQUENCY, RADIOFREQUENCY PULSES, RIGHT ANGLES, SOLUTIONS(GENERAL), SPECTROSCOPY, SYNTHESIS(CHEMISTRY), TENDONS
Subject Categories : Organic Chemistry
Distribution Statement : APPROVED FOR PUBLIC RELEASE