Accession Number : ADA189380

Title :   Metabolism, Mass Spectral Analysis and Mode of Action of Trichothecene Mycotoxins.

Descriptive Note : Annual rept. 15 Jul 86-14 Jul 87,

Corporate Author : MINNESOTA UNIV ST PAUL DEPT OF PLANT PATHOLOGY

Personal Author(s) : Mirocha, Chester J. ; Pawlosky, Robert J. ; Gunther, Roland

PDF Url : ADA189380

Report Date : 15 SEP 1987

Pagination or Media Count : 66

Abstract : The development of an analytical method for the analysis of T-2, HT-2 and T-2-tetraol in blood and urine was initiated. The objective is to use multiple reaction monitoring (MRM) facility of the VG 7070EQ tandem mass spectrometer. The advantages of the latter is that the noise from the biological matrix is kept at a minimum and as a result the sensitivity is increased. Essentially, the magnet is parked on the parent ion and only this fragment is allowed to passes into the quadrupole 1, it is decomposed into fragments by collision activated (in argon plasma and 20 30 volts) decomposition. The daughter ions are detected by quadrupole 2. Using this method, T-2 and HT-2 can be detected at one part per billion. Keywords: T-2 toxin; HT-2 Toxin.

Descriptors :   *MASS SPECTRA, *METABOLISM, *SPECTRUM ANALYSIS, *PHYTOTOXINS, *FUSARIUM, BLOOD, DECOMPOSITION, IONS, URINE, BIOASSAY.

Subject Categories : Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE