Accession Number : ADA189814

Title :   Cochlear Hair Cell Electrochemistry: Mechanisms for Bidirectional Transduction.

Descriptive Note : Annual progress rept. Jan-Dec 87,

Corporate Author : JOHNS HOPKINS UNIV BALTIMORE MD SCHOOL OF MEDICINE

Personal Author(s) : Brownell, William E

PDF Url : ADA189814

Report Date : 30 Dec 1987

Pagination or Media Count : 4

Abstract : Our objective is to determine the cellular mechanism responsible for cochlear outer hair cell (OHC) electromotility. Experiments are designed to test the working hypothesis that electrically evoked movements in OHCs result form electo-osmotic movement of cytoplasm in the outer hair cell's laminated cisternal system. More specifically, we postulate the intracellular potential gradients associated with acoustic transduction drive intracellular fluids through an electo-osmotic pump formed by the plasma and morphologically unique laminated cisternal membranes of the outer hair cells. The cell's cable properties are being measured to determine the range of intracellular potential gradients possible within OHC. The effect of temperature in the mechanical frequency response is being measured. The nature of the membrane surface charge is being probed with lectin binding experiments. The possibility of interaction between polyamines and the electrical double layer is being assessed.

Descriptors :   *COCHLEA, *ELECTROCHEMISTRY, *HAIR, AMINES, CELLS(BIOLOGY), ELECTRIC DOUBLE LAYER, EXTERNAL, FREQUENCY RESPONSE, GRADIENTS, HYPOTHESES, MEMBRANES(BIOLOGY), CYTOPLASM, STATIC ELECTRICITY

Subject Categories : Biochemistry
      Anatomy and Physiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE