Accession Number : ADA190111

Title :   Animal Studies in the Mode of Action of Agents, That Are Antitransformers in Cell Cultures.

Descriptive Note : Final rept. Jun 84-Aug 87,

Corporate Author : OESTERREICHISCHES FORSCHUNGSZENTRUM SEIBERSDORF G M B H VIENNA

Personal Author(s) : Altmann, Hans

PDF Url : ADA190111

Report Date : 28 Oct 1987

Pagination or Media Count : 114

Abstract : The ost important results of the animal studies on the mode of action of the antitransformer DADH on short and long term experiments are the following: Combined treatment of DADH and gamma-irradiation generated a decreased incidence of malignant lymphoma compared to gamma-irradiation alone. DADH itself shows some carcinogenic properties. In short term experiments DADH has an immunoprotective effect with respect to gamma-irradiation: a. earlier reconstitution of lymphocyte subsets, b. increase in natural killer cell activity. Higher poly(ADP-ribose)-polymerase activity to a certain extent seems to control replicative DNA synthesis and specific DNA amplification determined by double minutes. Spleen cells with loss of DNArepair increased remarkably with age. At the same time lymphoma incidence is increasing. Nucleotide sedimentation studies showed an oversedimentation phenomena rather than DNA breaks during short and long term experiments, A certain correlation between basic UDS in spleen cells and the occurrance of lymphomas exists. Basic UDS was highest in the combined (gamma + DADH) group. But also after a single irradiation dose of 1 Gy basic UDS was elevated during the whole life time. Poly(ADP-ribose)-polymerase activity parallels athe poly(ADP-ribose) content in spleen and liver cells at the end of the life span of C57 bl mice.

Descriptors :   *LYMPHOMAS, *CHEMOTHERAPY, *CHEMOTHERAPEUTIC AGENTS, AMPLIFICATION, BIOSYNTHESIS, CANCER, CARCINOGENS, CELLS(BIOLOGY), CULTURES(BIOLOGY), DEOXYRIBONUCLEIC ACIDS, IRRADIATION, LIFE SPAN(BIOLOGY), LIVER, LYMPHOCYTES, MICE, NUCLEOTIDES, SEDIMENTATION, SPLEEN, TIME, GAMMA RAYS, RADIATION DOSAGE

Subject Categories : Pharmacology
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE