Accession Number : ADA190127

Title :   Measurement of Pyridostigmine Bromide in Rodent Chow.

Descriptive Note : Final rept. 30 Jun-30 Sep 87,

Corporate Author : LETTERMAN ARMY INST OF RESEARCH PRESIDIO OF SAN FRANCISCO CA

Personal Author(s) : Ferraris, Suellen ; Ho, Bert ; Korte, Don W , Jr

PDF Url : ADA190127

Report Date : 19 Oct 1987

Pagination or Media Count : 17

Abstract : In order to evaluate the 180-day oral subchronic toxicity of pyridostigmine in rats, it was necessary to administer the test compound in the diet. Satisfactory incorporation of pyridostigmine bromide (PYR) into ground rat chow requires that the test substance be mixed uniformly at the correct concentration and remain stable in the feed for the desired time period. To assess these parameters, it was first necessary to develop an assay for PYR in rat chow and then use the assay to determine the homogeneity and stability of PYR mixed in ground rat chow. This report describes an assay for the quantitation of PYR in ground rat chow in which PYR was extracted from the chow with water and measured by High Performance Liquid Chromatography (HPLC) with UV detection. This assay was then used to assess the homogeneity and stability of PYR in rat chow which has been mixed in accordance with SOP OP-STX-106, Diet Preparation for Feeding Studies. The desired concentration of PYR in the chow for the feeding study and thus for this report ranged from 0.01 mg PYR/g to 2 mg PYR/g chow.

Descriptors :   *BROMIDES, *PYRIDINES, *CHOLINESTERASE INHIBITORS, *TOXIC TOLERANCES, *FOOD ADDITIVES, CARBAMATES, DIET, FEEDING, GROUND LEVEL, HIGH RESOLUTION, HYDROXYL RADICALS, LIQUID CHROMATOGRAPHY, MEASUREMENT, METHYL RADICALS, PREPARATION, RATS, ULTRAVIOLET DETECTORS, WATER, BIOASSAY, LABORATORY TESTS, RODENTS, LABORATORY ANIMALS

Subject Categories : Toxicology
      Pharmacology

Distribution Statement : APPROVED FOR PUBLIC RELEASE