Accession Number : ADA190313

Title :   Chemical Blistering: Cellular and Macromolecular Components.

Descriptive Note : Annual rept. 15 Sep 84-14 Sep 85,

Corporate Author : MICHIGAN UNIV ANN ARBOR

Personal Author(s) : Bernstein, I A ; Brabec, M J ; Conolly, R C ; Gray, R H ; Kulkarni, A

PDF Url : ADA190313

Report Date : 15 Nov 1987

Pagination or Media Count : 37

Abstract : The overall objective of this investigation is to elucidate the molecular mechanisms by which HD exerts its chemical blistering action when applied topically to human skin. The study involves the use of keratinocyte tissue cultures in order to focus on the direct interactions between the mustard and the cellular targets. The technical objectives of the project are to develop appropriate culture systems for use in the investigation of subcellular and macromolecular manifestations of toxicity, to establish the credibility of these systems for investigating the molecular mechanisms of these effects and to determine whether these systems can be used to develop procedures by which toxic responses can be neutralized. Keywords: Mustard, Keratinocyte, Tissue culture, Alkylation, Toxicity, Chemical blistering, Mitochondria, Metabolism, DNA repair, Epidermal proliferation, Epidermal differentiation.

Descriptors :   *CELLS(BIOLOGY), *TOXICITY, *MUSTARD AGENTS, DEOXYRIBONUCLEIC ACIDS, HUMANS, MACROMOLECULES, METABOLISM, MITOCHONDRIA, MOLECULAR PROPERTIES, SKIN(ANATOMY), TARGETS, TISSUE CULTURE, EXPOSURE(PHYSIOLOGY), RESPONSE(BIOLOGY), TISSUES(BIOLOGY)

Subject Categories : Chemical, Biological and Radiological Warfare

Distribution Statement : APPROVED FOR PUBLIC RELEASE