Accession Number : ADA190532

Title :   A Comparative Study Regarding the Association of Alpha-2U Globulin with the Nephrotoxic Mechanism of Certain Petroleum-Based Air Force Fuels.

Descriptive Note : Final rept. 1 Sep 86-31 Aug 87,

Corporate Author : ILLINOIS UNIV AT URBANA DEPT OF VETERINARY BIOSCIENCES

Personal Author(s) : Eurell, Thomas E

PDF Url : ADA190532

Report Date : 27 Oct 1987

Pagination or Media Count : 14

Abstract : Alpha-2U globulin is a low molecular weight urinary protein which may be associated with a hydrocarbon-induced proximal tubular cell degeneration in the male rat kidney. A new method was developed to obtain monospecific immunologic reagents for alpha-2U globulin using diafiltration, anion-exchange and hydroxylapatite chromatography. Isoelectric focusing techniques were developed to isolate the major isoelectric variants of the alpha-2U globulin molecule and to assess changes in alpha-2U globulin after experimental exposure to hydrocarbon compounds. Alpha-2U globulin was isolated from the urine of albino and pigmented male rats to study strain susceptibility to the nephrotoxic process. An alpha-2u globulin isoelectric variant profile distinguishing albino from non-albino male rats was not apparent, however, strain differences were revealed. Fischer 344 male rats appear to have higher levels of the isoelectric variants than the other strains studied. These findings suggest that if a strain susceptibility to the hydrocarbon-induced nephrotoxic lesion exists, it may be associated with the alpha-2U globulin isoelectric variant profile.

Descriptors :   *FUELS, *HYDROCARBONS, *KIDNEYS, *GLOBULINS, *TOXICITY, *BIOASSAY, AIR FORCE, CELLS(BIOLOGY), CHEMICAL AGENTS, IMMUNOLOGY, MALES, PETROLEUM PRODUCTS, RATS, URINE, EXPOSURE(PHYSIOLOGY), LESIONS, ANTIBODIES, TOXIC TOLERANCES

Subject Categories : Biochemistry
      Toxicology
      Fuels

Distribution Statement : APPROVED FOR PUBLIC RELEASE