Accession Number : ADA191772
Title : Hyaluronidase: Purification and Inhibition by a Gold Complex and a Steroid Derivative.
Descriptive Note : Master's thesis,
Corporate Author : AUBURN UNIV AL
Personal Author(s) : Durante, Gary G
PDF Url : ADA191772
Report Date : 11 Dec 1987
Pagination or Media Count : 72
Abstract : The enzyme hyaluronidase from human synovial fluid was partially purified using gel filtration and chromotography on a hydroxylapatite column. A 4.1 fold purification of hyaluronidase having a specific activity of 1.1 x 10 to the minus 4th power micro mol/min/mg was obtained. The enzyme was inhibited by the gold complex Auranofin, and the steroid derivative, 22 ketocholesterol oxime. It was shown that the complexes differed in their ability to inhibit the enzyme. The rate constants and equilibrium inhibition constants were calculated. Kinetic analyses demonstrated an apparent uncompetitive inhibition by the steroid derivative 22-ketocholesterol oxime.
Descriptors : *GLYCOSIDE HYDROLASES, *ENZYME INHIBITORS, *ARTHRITIS, EQUILIBRIUM(GENERAL), FILTRATION, GELS, GOLD, INHIBITION, OXIMES, PURIFICATION, STEROIDS, THESES, BODY FLUIDS, IMMUNOLOGY, BIOASSAY, REACTION KINETICS
Subject Categories : Biochemistry
Medicine and Medical Research
Distribution Statement : APPROVED FOR PUBLIC RELEASE