Accession Number : ADA191939

Title :   Quantitation of Nitroguanidine in Rodent Chow,

Corporate Author : LETTERMAN ARMY INST OF RESEARCH PRESIDIO OF SAN FRANCISCO CA

Personal Author(s) : Wheeler, Conrad ; Dahlberg, Eric ; Ryabik, John ; Korte, Don W , Jr

PDF Url : ADA191939

Report Date : Jan 1988

Pagination or Media Count : 13

Abstract : The assay described in this report was developed to verify the concentrations of nitroguanidine (NGu) in feed mixtures used in GLP animal studies. The target concentrations of NGu in the animal diets ranged from approximately 1 to 22 mg NGu/g feed. These diets were prepared using a mixture of feed (premix) with a much higher concentration of NGu (approximately 50 mg NGu/g feed). The assay employs High Pressure Liquid Chromatography (HPLC) and is suitable for determining NGu over this entire range of concentrations. Using methylnitroguanidine (MNGu) as an internal standard, weighed amounts of the animal diets were diluted, filtered and analyzed by HPLC. The peak ratios were then used to quantitate the concentration of NGu in the final dilution. Sample weights and dilution factors permit the calculation of the NGu concentration in the original feed mixture.

Descriptors :   *FEEDING, *NITROGUANIDINE, *FOOD, *QUANTITATIVE ANALYSIS, *AGRICULTURAL CHEMISTRY, ANIMALS, DIET, DILUTION, HIGH PRESSURE, INTERNAL, LIQUID CHROMATOGRAPHY, MIXTURES, PEAK VALUES, RATIOS, RODENTS, STANDARDS

Subject Categories : Agricultural Chemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE