Accession Number : ADA192078

Title :   Chemical Synthesis Coxiella Burnetti Lipopolysaccharides: Structural Studies of Coxiella Burnetti Lipopolysaccharides.

Descriptive Note : Final rept. 30 Sep 85-29 Sep 87; annual rept. 30 Sep 86-29 Sep 87,

Corporate Author : HARVARD SCHOOL OF PUBLIC HEALTH BOSTON MASS

Personal Author(s) : Her, G R ; Reinhold, V N

PDF Url : ADA192078

Report Date : 30 Nov 1987

Pagination or Media Count : 61

Abstract : Lipopolysaccharide (LPS) materials were extracted from C. burnetii (CB9MI514 & CB9MIIC4) with a modified phenol-water procedure, purified by ultracentrifugation, and forwarded to this facility for structural characterization. The LPS was degraded by partial acid hydrolysis and separated into lipophilic and hydrophilic fractions with the designations, Lipid C and O-Antigen, respectively. Preliminary structural data has been obtained for Lipid C and the O-Antigen of CB9MI514 and more confirmatory data and corroborative evidence is provided for the structure of O-Antigen as well as new information on the Lipid C of CB9MIIC4. Sample permethylation on intact LPS (CB9MIIC4) by differential CD3/CH3 analysis has provided an approximation of molecular weight. Supercritical fluid chromatography (SFC) and SFC-MS studies have been initiated for application to this problem. Keywords: Toxicity; Immunology; Molecular structure.

Descriptors :   *LIPOPOLYSACCHARIDES, *MOLECULAR WEIGHT, *VACCINES, *IMMUNOGENS, *COXIELLA BURNETII, ACIDS, CHROMATOGRAPHY, FLUIDS, HYDROLYSIS, IMMUNOLOGY, LIPIDS, MOLECULAR STRUCTURE, PURIFICATION, SUPERCRITICAL FLOW, SYNTHESIS(CHEMISTRY), TOXICITY, ULTRACENTRIFUGES

Subject Categories : Genetic Engineering and Molecular Biology
      Pharmacology
      Microbiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE