Accession Number : ADA192090

Title :   Final Technical Report ONT/ASEE (Office of Naval Technology/American Society for Engineering Education) Postdoctoral Fellow on Contract N00014-83-D-0689.

Descriptive Note : Rept. for 1 Mar 86-30 Nov 87,

Corporate Author : AMERICAN SOCIETY FOR ENGINEERING EDUCATION WASHINGTON DC

Personal Author(s) : Ladouceur, Cynthia A

PDF Url : ADA192090

Report Date : 19 Feb 1988

Pagination or Media Count : 37

Abstract : This final technical report consists of two technical papers which were submitted for publication. The first paper discusses Indium-Coated Membranes Versus Nitrocellulose Membranes for use in a Spot Immunoassay. Immunoassays performed on membranes can be used for the detection of large or small molecular weight substances. The end product of the immunoassay is a spot on the surface of the membrane which is discernable with the naked eye. We tested three different enzyme-substrate systems and colloidal gold with silver amplification. The resulting spots varied in color and in their ability to resist fading. When indium-coated membranes are used, antigen-antibody reactions can be visualized with or without development reagents. With amplification, the quality of the spots produced on nitrocellulose membranes is far superior to the spots produced on indium-coated membranes. The second paper pertains to technologies used in mass screening for drugs of abuse. Four mass screening techniques for drugs of abuse Thin Layer Chromatography, Radioimmunoassay, Enzyme Multiple Immunoassay Technique and Fluorescent Polarization Immunoassay have been described. For small-scale screening, TLC is the most cost effective.

Descriptors :   *ANTIGEN ANTIBODY REACTIONS, *IMMUNOASSAY, *MEMBRANES, *BIOASSAY, AMPLIFICATION, CHEMICAL AGENTS, COLLOIDS, COSTS, DETECTION, DRUGS, ENZYMES, EYE, FLUORESCENCE, GOLD, MASS, MOLECULAR WEIGHT, NITROCELLULOSE, POLARIZATION, RADIOIMMUNOASSAY, SILVER, SUBSTRATES, THIN LAYER CHROMATOGRAPHY, HISTOLOGICAL TECHNIQUES, URINE, DRUG ABUSE

Subject Categories : Biochemistry
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE