Accession Number : ADA192224

Title :   Ischemic-Anoxia of the Central Nervous System: Iron Dependent Oxidative Injury during Reperfusion.

Descriptive Note : Annual rept. 1 Sep 84-31 Aug 85,

Corporate Author : MICHIGAN STATE UNIV EAST LANSING

Personal Author(s) : White, Blaine C ; Krause, Gary S ; Aust, Steven D ; Indrieri, Richard J ; Evans, A T

PDF Url : ADA192224

Report Date : 30 Sep 1985

Pagination or Media Count : 42

Abstract : This work has been undertaken to systematically characterize the contribution and time course of membrane damage by lipid peroxidation in the brain during cardiac arrest, resuscitation, and in the post-resuscitation care phase, and to develop effective interventions during resuscitation and post-resuscitation care to prevent biochemical, structural, and functional neurologic injury. It is now generally recognized that lipid peroxidation will not occur without catalysis by a transitional metal such as iron. We have used a 15 minute cardiac arrest model in the dog with resuscitation by internal cardiac massage and defibrillation for our biochemical studies. Intervention in the post-resuscitation phase with the potent iron chelator deferoxamine in a dose of 50 mg/kg IV returns tissue concentrations of lipid peroxidation products to pre-ischemic levels after 2 hours of reperfusion. We have also studied the effect of different artificial perfusion techniques on iron delocalization during a 30 minute resuscitation period following a 15 minute cardiac arrest. We also carried out a preliminary prospective and blind outcome trial in which treatment with both deferoxamine and lidoflazine (a calcium antagonist) during the post resuscitation care phase were compared to standard intensive care without specific drug intervention.

Descriptors :   *BRAIN, *CARDIAC ARREST, *RESUSCITATION, *ANOXIA, *ISCHEMIA, BIOCHEMISTRY, CALCIUM, CATALYSIS, CENTRAL NERVOUS SYSTEM, DAMAGE, DEFIBRILLATION, DOGS, DRUGS, INTERVENTION, IRON, LIPIDS, MEDICAL SERVICES, MEMBRANES, MODELS, NERVOUS SYSTEM DISEASES, OXIDATION, PERFUSION, WOUNDS AND INJURIES, CHELATING AGENTS, PHARMACOLOGICAL ANTAGONISTS

Subject Categories : Medicine and Medical Research
      Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE