Accession Number : ADA192386
Title : Pressure Studies of Protein Dynamics.
Descriptive Note : Annual rept. 1 Mar 87-26 Feb 88,
Corporate Author : ILLINOIS UNIV AT URBANA DEPT OF PHYSICS
Personal Author(s) : Frauenfelder, Hans ; Young, Robert D
PDF Url : ADA192386
Report Date : 26 Feb 1988
Pagination or Media Count : 20
Abstract : In this research we extend and deepen our studies of the relation between dynamic structure and function in proteins. We study protein dynamics using flesh photolysis together with near ultraviolet, visible, and near and mid-infrared spectroscopies over wide ranges in time (50 ns-10 ks), temperature (60-320K), and pressure (0.1-100 MPa). Initially we study a simple biomolecular reaction -- carbon monoxide (CO) binding to myoglobin (Mb). We have greatly expanded knowledge of pressure effects on proteins by using the infrared CO stretching frequencies in MbCO as a probe. The combined pressure and temperature studies shed new light on various features of the hierarchical model of protein substates and motions. These studies also provide much information on the glass like behavior of proteins, including the slaved glass transition and glass-like relaxation processes near the transition temperature. Keywords: Protein dynamics, Pressure, Myoglobin, Hierarchy of substates, Flash photolysis, Carbon dioxide(CO) stretching bands, Glass like properties.
Descriptors : *CARBON MONOXIDE, *PROTEINS, *CHEMICAL BONDS, *CHEMICAL REACTIONS, *HEMOGLOBIN, DYNAMICS, FLASHES, GLASS, HIERARCHIES, INTERMEDIATE INFRARED RADIATION, MODELS, NEAR ULTRAVIOLET RADIATION, PHOTOLYSIS, PRESSURE, RANGE(EXTREMES), SPECTROSCOPY, TRANSITION TEMPERATURE, LIGANDS, BIOMOLECULES, MOLECULAR STRUCTURE, MOLECULAR STATES, BIOPHYSICS
Subject Categories : Biochemistry
Distribution Statement : APPROVED FOR PUBLIC RELEASE