Accession Number : ADA192751

Title :   Isolation of Genomic Clone for Human Cholinesterase.

Descriptive Note : Annual summary rept. 15 Nov 85-15 Nov 86,

Corporate Author : MICHIGAN UNIV ANN ARBOR DEPT OF PHARMACOLOGY

Personal Author(s) : Lockridge, Oksana

PDF Url : ADA192751

Report Date : Dec 1986

Pagination or Media Count : 30

Abstract : The goal of this work is to isolate the gene for human serum cholinesterase (EC 3.1.1.8). We have succeeded in isolating a partial clone that codes for amino acids phenylalanine 195 through leucine 574, and contains 302 bp of the 3' untranslated region. This clone was found by screening a human brain Complementary Deoxyribonucleic Acid (cDNA) library with oligonucleotide probes. The probes corresponded to the amino sequence of human serum cholinesterase. The coding region of our 1440 bp clone matched the amino acid sequence of human serum cholinesterase perfectly. This suggested that human cholinesterase was identical in brain and in serum. We are continuing to search the brain cDNA library for other cDNA clones that will extend our present clone in the 5' direction so as to have the complete coding region of cholinesterase. Keywords: Pseudocholinesterase, Genetic engineering, Recombinant DNA, Gene sequencing.

Descriptors :   *AMINO ACIDS, *CHOLINESTERASE, *CLONES, *DEOXYRIBONUCLEIC ACIDS, *GENETIC ENGINEERING, *BIOMEDICINE, AMINES, BLOOD SERUM, BRAIN, PURIFICATION, NUCLEOTIDES

Subject Categories : Biochemistry
      Genetic Engineering and Molecular Biology
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE