Accession Number : ADA192945

Title :   Paradaxin's Action in Shark.

Descriptive Note : Progress rept.,

Corporate Author : NEW YORK AQUARIUM BROOKLYN OSBORN LABS OF MARINE SCIENCES

Personal Author(s) : Primor, Naftali ; Lazarovici, Philip

PDF Url : ADA192945

Report Date : 26 Feb 1988

Pagination or Media Count : 4

Abstract : The objectives of this research were: (1) In Vivo binding study of pardaxin (PX) to gill membranes, (2) Testing for activities of a synthetic pardaxin and N-terminal decapeptide and 26 amino acid peptide, and (3) Permeabilities for nonvalent and divalent cations in pardaxin channels in lipid bilayer membranes. In experiments in which we tested the effect of proteinase K (PK) and pronase on PX binding and toxicity, the enzyme was first added to the medium for 20 min. Then the fish were washed and H-PX with and without unlabelled PX was added to the medium for 30 min. The effect of unlabelled PX on the binding of H-PX and toxicity is given.

Descriptors :   *FISH GILLS, *IN VIVO ANALYSIS, *MEMBRANES, ENZYMES, LAYERS, LIPIDS, TOXICITY

Subject Categories : Biochemistry
      Biology

Distribution Statement : APPROVED FOR PUBLIC RELEASE