Accession Number : ADA193104

Title :   Effects of Pulsed and CW (Continuous Wave) 2450 MHz Radiation on Transformation and Chromosomes of Human Lymphocytes in Vitro.

Descriptive Note : Progress rept. 1 Oct 86-1 Oct 87,

Corporate Author : MARYLAND UNIV COLLEGE PARK DEPT OF ELECTRICAL ENGINEERING

Personal Author(s) : Davis, Christopher C

PDF Url : ADA193104

Report Date : 16 Feb 1988

Pagination or Media Count : 47

Abstract : The main objective of this project is to determine conditions of exposure of human lymphocytes in vitro to continuous wave (CW) or pulsed wave (PW) 2450 MHz microwaves, which: affect the course of spontaneous or phytohemagglutin in (PHA)-induced lymphoblastoid transformation, and may induce numerical and/or structural aberrations in chromosomes of cells undergoing division following transformation. In order to provide reliable quantitative data on exposure, a system will automated dosimetry was developed, and tested for biocompatibility with lymphocyte cultures in vitro for periods of up to 120 hrs. (5 days). A method for quantitation of lymphocyte transformation based on image analysis was developed and tested. Experiments on effects of exposure on spontaneous transformation were carried out. For convenience and clarity the work performed is described under two separate headings: (1) exposure system and dosimetry, and (2) studies on lymphocyte cultures.

Descriptors :   *CHROMOSOMES, *LYMPHOCYTES, *MICROWAVES, *RADIATION EFFECTS, AUTOMATION, CONTINUOUS WAVES, DISTORTION, DOSIMETRY, HUMANS, IMAGE PROCESSING, IN VITRO ANALYSIS, PULSES, RADIATION, RELIABILITY, TRANSFORMATIONS, WAVES, TISSUE CULTURE CELLS, TEMPERATURE, HEMOGLOBIN, AGGLUTININS, CELL DIVISION

Subject Categories : Stress Physiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE