Accession Number : ADA193468

Title :   Biological Synthesis of a Protein Analogue of Acetylcholinesterase: Monoclonal Anti-Idiotype Antibody Analogue of the Esteratic Site.

Descriptive Note : Annual rept. 15 May 85-14 May 86,

Corporate Author : JOHNS HOPKINS UNIV BALTIMORE MD SCHOOL OF MEDICINE

Personal Author(s) : August, J T

PDF Url : ADA193468

Report Date : 10 Jul 1986

Pagination or Media Count : 16

Abstract : We have studied the binding properties and inhibitory activity of AE-2, a monoclonal antibody which binds to acetylcholinesterase(AChE) and inhibits its activity by up to 50%. This antibody was compared to C1B7, another inhibitory enzyme. It was determined that these antibodies bind to different sites on AChE; these sites were distinct from the active site and from the binding sites for a number of anticholinergic drugs, including DFP, propidium, endrophonium and neostigmine. A kinetic analysis of inhibition by AE-2 and C1B7 suggested that their inhibition of AChE was non-competitive and allosteric.

Descriptors :   *ACETYLCHOLINESTERASE, *ANTIBODIES, *CHOLINESTERASE INHIBITORS, *CLONES, *BIOSYNTHESIS, ENZYMES, INHIBITION, KINETICS, PARASYMPATHOMIMETIC AGENTS, PROTEINS, SITES, CHEMICAL ANALOGS

Subject Categories : Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE