Accession Number : ADA193468
Title : Biological Synthesis of a Protein Analogue of Acetylcholinesterase: Monoclonal Anti-Idiotype Antibody Analogue of the Esteratic Site.
Descriptive Note : Annual rept. 15 May 85-14 May 86,
Corporate Author : JOHNS HOPKINS UNIV BALTIMORE MD SCHOOL OF MEDICINE
Personal Author(s) : August, J T
PDF Url : ADA193468
Report Date : 10 Jul 1986
Pagination or Media Count : 16
Abstract : We have studied the binding properties and inhibitory activity of AE-2, a monoclonal antibody which binds to acetylcholinesterase(AChE) and inhibits its activity by up to 50%. This antibody was compared to C1B7, another inhibitory enzyme. It was determined that these antibodies bind to different sites on AChE; these sites were distinct from the active site and from the binding sites for a number of anticholinergic drugs, including DFP, propidium, endrophonium and neostigmine. A kinetic analysis of inhibition by AE-2 and C1B7 suggested that their inhibition of AChE was non-competitive and allosteric.
Descriptors : *ACETYLCHOLINESTERASE, *ANTIBODIES, *CHOLINESTERASE INHIBITORS, *CLONES, *BIOSYNTHESIS, ENZYMES, INHIBITION, KINETICS, PARASYMPATHOMIMETIC AGENTS, PROTEINS, SITES, CHEMICAL ANALOGS
Subject Categories : Biochemistry
Distribution Statement : APPROVED FOR PUBLIC RELEASE