Accession Number : ADA193676

Title :   Molecular Biology of the Extremely Thermophilic Archaebacterium, Methanothermus Fervidus.

Descriptive Note : Annual rept. Apr 87-Mar 88,

Corporate Author : OHIO STATE UNIV COLUMBUS DEPT OF MICROBIOLOGY

Personal Author(s) : Reeve, John N

PDF Url : ADA193676

Report Date : 15 Apr 1988

Pagination or Media Count : 10

Abstract : Genes encoding the subunit polypeptides of methyl coenzyme M reductase, tRNAs and rRNAs have been cloned and sequenced from the extremely thermophilic archaebacterium Methanothermus fervidus. The primary sequences of these genes have been compared with the functionally equivalent sequences from mesophilic and less thermophilic methanogens. Highly conserved regions and structural features which may play a role in thermostability have been identified. A DNA binding protein (HMf) has been purified from M. fervidus which, when bound to double-stranded DNA increases the temperature of its thermal denaturation, in vitro, by as much as 40 degrees.

Descriptors :   *MOLECULAR BIOLOGY, *BACTERIA, CHEMICAL BONDS, CLONES, CODING, DEOXYRIBONUCLEIC ACIDS, GENES, IN VITRO ANALYSIS, PEPTIDES, POLYMERS, SEQUENCES

Subject Categories : Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE