Accession Number : ADA193723
Title : Cloning and Production of Human Acetylcholinesterase.
Descriptive Note : Final rept. 1 May 82-30 Apr 84,
Corporate Author : ARIZONA UNIV TUCSON DEPT OF MOLECULAR AND CELLULAR BIOLOGY
Personal Author(s) : Aposhian, V V
PDF Url : ADA193723
Report Date : 15 Jan 1986
Pagination or Media Count : 14
Abstract : The goal of this work was to clone by DNA recombinant technology the gene for acetylcholinesterase (EC 188.8.131.52) from a human source in order to be able to produce large amounts of pure enzyme under the direction of a gene from a single source. Unfortunately, our attempts were unsuccessful. Lack of success was probably due to the acetylcholinesterase mNRA being present in such small amounts and available techniques not being good enough to detect such minute amounts of messenger. Human neuroblastoma SK-N-SH cells were grown in culture and used in these studies. In retrospect, it appears that seeking cells that produce more than normal amounts of human acetylcholinesterase (HACE) and using modern protein purification procedures might be a better approach to obtain HACE.
Descriptors : *ACETYLCHOLINESTERASE, *CLONES, *BIOSYNTHESIS, GENETIC ENGINEERING, HUMANS, PRODUCTION, PROTEINS, PURIFICATION, PURITY, SOURCES
Subject Categories : Genetic Engineering and Molecular Biology
Distribution Statement : APPROVED FOR PUBLIC RELEASE