Accession Number : ADA193746

Title :   Protein Structure and Function: Design and Synthesis of Peptide Recognition Sequences.

Descriptive Note : Annual rept. 1 May 87-30 Apr 88,

Corporate Author : JOHNS HOPKINS UNIV BALTIMORE MD DEPT OF PHARMACOLOGY

Personal Author(s) : August, Thomas J ; Amzel, Mario L ; Mildvan, Albert S ; Robinson, Cecil H

PDF Url : ADA193746

Report Date : 18 May 1988

Pagination or Media Count : 4

Abstract : The long-range goal of this research is to elucidate the molecular mechanism of protein-protein recognition and binding. The immediate goal is to undertake the synthesis and chemical modification of model peptide ligands, to characterize the structure of these peptides through NMR spectroscopy and x-ray crystallography, and to test their function in in vitro and in vivo assay systems. We also propose to identify and characterize the primary and secondary structure of the cell membrane receptor(s) of the ligands. Models of cell membrane receptor-peptide ligand binding will be constructed from structural studies of the purified receptor, NMR analysis of ligand binding to the receptor, and from monoclonal antibody surrogates reacting with the peptide active site.

Descriptors :   *PEPTIDES, *PROTEINS, *MOLECULE MOLECULE INTERACTIONS, *MOLECULAR BIOLOGY, ANTIBODIES, CELLS(BIOLOGY), CHEMICAL PROPERTIES, CLONES, CRYSTALLOGRAPHY, IN VITRO ANALYSIS, IN VIVO ANALYSIS, LIGANDS, MEMBRANES(BIOLOGY), MODELS, MODIFICATION, MOLECULES, PURIFICATION, RECOGNITION, SECONDARY, SENSE ORGANS, SEQUENCES, SITES, SPECTROSCOPY, X RAYS, SYNTHESIS(CHEMISTRY)

Subject Categories : Genetic Engineering and Molecular Biology
      Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE