Accession Number : ADA194117

Title :   Stable Isotope Technology Applied to Controlled Dehydration, Rate of Entry, and Water Turnover Studies in Humans.

Descriptive Note : Final rept. 1 Mar 86-28 Feb 87,

Corporate Author : BOSTON UNIV MA SCHOOL OF MEDICINE

Personal Author(s) : Janghorbani, Morteza

PDF Url : ADA194117

Report Date : Sep 1987

Pagination or Media Count : 38

Abstract : Three experiments were conducted in human subjects: (1) Investigation of sampling protocols for optimum sampling in studies using water as metabolic tracer; (2) An experiment designed to obtain initial data on changes in Total Body Water (TBW) consequent to experimental dehydration and to define conditions for sequential measurement of TBW. and (3) An initial experiment addressing the relationship between rate of absorption of water and composition of drinking solution. It was found that use of mixed saliva did not permit accurate sampling due to problems of contamination with exogenous water, while a special device (Curby cup) was very effective for collection of parotoid saliva. While not effective for studies of rate of absorption of water, urine was a suitable sampling medium for measurement of TBW. Sequential measurement of TBW required use of special correction procedure for drifting baseline. This was found to be an important experimental problem in relation to accurate measurement of small decrements in TBM. Data obtained indicated that rate of absorption of water from drinks containing significant concentrations of carbohydrate/electrolytes did not differ consistently from that for pure water.

Descriptors :   *BODY FLUIDS, *BIOLOGICAL ABSORPTION, *WATER ABSORPTION TESTS, *DRINKING WATER, BASE LINES, CONTAMINATION, DEGRADATION, DEHYDRATION, DRIFT, ISOTOPES, MEASUREMENT, MIXING, OPTIMIZATION, PURITY, SALIVA, SAMPLING, URINE, WATER, WATER ENTRY, WATER EXIT, WATER CHEMISTRY, TRACER STUDIES

Subject Categories : Anatomy and Physiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE